Journal: Nanomaterials

Article Title: PDMS Nano-Modified Scaffolds for Improvement of Stem Cells Proliferation and Differentiation in Microfluidic Platform

doi: 10.3390/nano10040668

Figure Lengend Snippet: Normalized optical density measurement of calcium deposition of hAMSCs differentiated to osteogenic lineage on nanoparticle (NP)/PDMS substrate ( a , b ). Mean fluorescence intensity measurement of immunofluorescence staining of Coll I of hAMSCs differentiated to osteogenic lineage on NP/PDMS substrates ( c , d ).

Article Snippet: A certain amount of cells per mL (based on the surface area of the cell culture chamber) were seeded into the microfluidics system, and after expansion, the hAMSCs medium was replaced with osteogenic stem cell differentiation medium (StemMACS OsteoDiff Media, order No. 130-091-678, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) supplemented with FCS 10% and 1% penicillin/streptomycin.

Techniques: Fluorescence, Immunofluorescence, Staining

Journal: Stem Cell Research & Therapy

Article Title: JNKi- and DAC-programmed mesenchymal stem/stromal cells from hESCs facilitate hematopoiesis and alleviate hind limb ischemia

doi: 10.1186/s13287-019-1302-1

Figure Lengend Snippet: The hESC-MSCs exhibit signatures of adult MSCs but superiority in long-term proliferation. a Adipogenic differentiation potential of hESC-MSCs and hBM-MSCs was identified by Oil red O staining (scale bar = 100 μm). b qRT-PCR analysis of the adipogenic markers ( ADIPOQ , PPAR-γ ) in hESC-MSCs and hBM-MSCs. Data are shown as mean ± SEM ( n = 3). * P < 0.05; ** P < 0.01. All values are normalized to the hBM-MSC group (= 1). c Osteogenic differentiation potential of hESC-MSCs and hBM-MSCs was identified by Alizarin red staining (scale bar = 100 μm). d qRT-PCR analysis of the osteogenic markers ( RUNX2 , BGLAP ) in hESC-MSCs and hBM-MSCs. Data are shown as mean ± SEM ( n = 3). * P < 0.05. All values are normalized to the hBM-MSC group (= 1). e Chondrogenic differentiation potential of hESC-MSCs and hBM-MSCs was identified by Alcian blue staining (scale bar = 100 μm). f qRT-PCR analysis of the chondrogenic markers ( ACAN , SOX9 ) in hESC-MSCs and hBM-MSCs. Data are shown as mean ± SEM ( n = 3). NS, not significant. All values are normalized to the hBM-MSC group (= 1). g Karyotype analysis of hESC-MSCs and hBM-MSCs with G-banded chromosome experiment. h Expansion potential of hESC-MSCs and hBM-MSCs for 12 passages by population doubling assay (Pd). Data are shown as mean ± SEM ( N = 3)

Article Snippet: For induction of chondrogenic differentiation, hESC-MSCs and hBM-MSCs were seeded at a density of 5 × 10 3 cells/cm 2 and cultured in chondrogenic differentiation medium (MesenCultTM-ACF Chondrogenic Differentiation Kit, Stem Cell Technologies) and the medium was changed every 3 days.

Techniques: Staining, Quantitative RT-PCR

Journal: Journal of Tissue Engineering

Article Title: Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders

doi: 10.1177/20417314211001545

Figure Lengend Snippet: Identification of Ad-MSCs (a–c) and AMEs (d–f): (a) characterization of the fourth generation of Ad-MSCs was performed by flow cytometry, and the Ad-MSC histogram revealed that after purification, the cells mostly expressed mesenchymal cell markers. Differentiation assays showed that Ad-MSCs could differentiate into adipocytes (b) and osteocytes (c), which were stained with Oil Red O and Von Kossa, respectively. Scale bar = 100 μm. (d) TEM of exosomes isolated from MSCM-cultured Ad-MSCs for 3 days with 10% exosome-depleted FBS. Scale bars = 100 nm. (e) NTA found that most of these vesicles ranged in diameter from 30 to 150 nm. (f) Western blotting was performed with Ad-MSCs (MSCs) and AMEs (Exos). The expression of CD9, CD63, TSG101, and calnexin was detected. Ad-MSCs: adipose-derived mesenchymal stem cells; AMEs: adipose-derived mesenchymal stem cell exosomes; NTA: nanoparticle tracking analysis; TEM: transmission electron microscopy.

Article Snippet: The medium was changed to mesenchymal stem cell adipogenic differentiation medium (MADM, ScienCell) and mesenchymal stem cell osteogenic differentiation medium (MODM, ScienCell) when the cells reached 70–80% confluency.

Techniques: Flow Cytometry, Purification, Staining, Isolation, Cell Culture, Western Blot, Expressing, Derivative Assay, Transmission Assay, Electron Microscopy

Journal: Journal of Tissue Engineering

Article Title: Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders

doi: 10.1177/20417314211001545

Figure Lengend Snippet: Identification of Ad-MSCs (a–c) and AMEs (d–f): (a) characterization of the fourth generation of Ad-MSCs was performed by flow cytometry, and the Ad-MSC histogram revealed that after purification, the cells mostly expressed mesenchymal cell markers. Differentiation assays showed that Ad-MSCs could differentiate into adipocytes (b) and osteocytes (c), which were stained with Oil Red O and Von Kossa, respectively. Scale bar = 100 μm. (d) TEM of exosomes isolated from MSCM-cultured Ad-MSCs for 3 days with 10% exosome-depleted FBS. Scale bars = 100 nm. (e) NTA found that most of these vesicles ranged in diameter from 30 to 150 nm. (f) Western blotting was performed with Ad-MSCs (MSCs) and AMEs (Exos). The expression of CD9, CD63, TSG101, and calnexin was detected. Ad-MSCs: adipose-derived mesenchymal stem cells; AMEs: adipose-derived mesenchymal stem cell exosomes; NTA: nanoparticle tracking analysis; TEM: transmission electron microscopy.

Article Snippet: The medium was changed to mesenchymal stem cell adipogenic differentiation medium (MADM, ScienCell) and mesenchymal stem cell osteogenic differentiation medium (MODM, ScienCell) when the cells reached 70–80% confluency.

Techniques: Flow Cytometry, Purification, Staining, Isolation, Cell Culture, Western Blot, Expressing, Derivative Assay, Transmission Assay, Electron Microscopy